IntroductionBananas are widely grown in Malaysia as they are in high demand, it is ranked as the second largest fruit crop produced in Malaysia. Bananas are healthy to consume as they contain high vitamins and minerals (Ware, 2014). However, banana production in our country has declined due to several factors, including the threat of various diseases, high production costs and lack of diversified planting materials. Jong (2012) stated that about 50% of banana plantations in Malaysia are grown mainly for export, such as Pisang Cavendish. The conventional banana planting method involves growing bananas from their vegetative suckers. However, this is not considered an ideal method in banana production as planting materials can carry viruses and fungal pathogens (Jong, 2012). Therefore, tissue culture is the alternative to the conventional method for banana production. The tissue culture method benefits banana production by producing plants that are disease-free, faster growth rates, higher yield, and safe for human consumption (Increasing Banana Yield Using Tissue Culture Method, n.d.). According to Tissue Culture (n.d.), the main purpose of tissue culture is to produce healthy, viral disease-free seedlings with high yields. Micropropagation is one of the plant tissue culture techniques commonly used in banana tissue culture production. According to Biotechnology: Tissue Culture (n.d.), micropropagation is defined as the production of whole plants from explant-derived cell cultures. It can be further divided into shoot culture, organogenesis, and somatic embryogenesis. Tip culture is the most commonly used method for commercial micropropagation. It has the main advancement...... half of the paper.......). Tissue culture banana plant productions are uniform and can be supplied at any time of the year, regardless of the season. Conventional agriculture depends on the weather and seasons. In addition to this, conventional agriculture is not uniform as some banana plants mature faster than others, resulting in increased production costs (Tissue Culture, n.d.). Transporting tissue culture bananas is also cheaper and easier than conventional farming. Although bananas produced by micropropagation have many advantages over the conventional method, tissue culture is considered an expensive method with high production costs. Therefore, it is important to develop a low-cost micropropagation technique to make it accessible to buyers, especially farmers, without compromising the quality of the plants produced..